Retraction: Chicory (Cichorium intybus L.) Root Extract Regulates the Oxidative Status and Antioxidant Gene Transcripts in CCl4-Induced Hepatotoxicity
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چکیده
The ability of Cichorium intybus root extract (chicory extract) to protect against carbon tetrachloride (CCl4)-induced oxidative stress and hepatotoxicity was evaluated in male rats. The rats were divided into four groups according to treatment: saline (control); chicory extract (100 mg/kg body weight daily, given orally for 2 weeks); CCl4 (1 ml/kg body weight by intraperitoneal injection for 2 consecutive days only); or chicory extract (100 mg/kg body weight daily for 2 weeks) + CCl4 injection on days 16 and 17. The levels of hepatic lipid peroxidation, antioxidants, and molecular biomarkers were estimated twenty-four hours after the last CCl4 injection. Pretreatment with chicory extract significantly reduced CCl4-induced elevation of malondialdehyde levels and nearly normalized levels of glutathione and activity of glutathione S-transferase, glutathione peroxidase (GPx), glutathione reductase, catalase (CAT), paraoxonase-1 (PON1), and arylesterase in the liver. Chicory extract also attenuated CCl4-induced downregulation of hepatic mRNA expression levels of GPx1, CAT and PON1 genes. Results of DNA fragmentation support the ability of chicory extract to ameliorate CCl4-induced liver toxicity. Taken together, our results demonstrate that chicory extract is rich in natural antioxidants and able to attenuate CCl4-induced hepatocellular injury, likely by scavenging reactive free radicals, boosting the endogenous antioxidant defense system, and overexpressing genes encoding antioxidant enzymes.
منابع مشابه
Hepatoprotective Activity of Cichorium intybus L. Leaves Extract Against Carbon Tetrachloride Induced Toxicity
The effects of different concentrations of the hydroalcoholic extract of dried powdered leaves of Cichorium intybus L., on CCl4-induced hepatotoxicity in vivo in rats and CCl4-induced cytotoxicity in isolated rat hepatocytes were investigated. Rats received different concentrations of the extract by i.p. injection for 3 consecutive days before the injection of (3ml/kg) CCl4 (i.p.). Twenty four ...
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The effects of different concentrations of the hydroalcoholic extract of dried powdered leaves of Cichorium intybus L., on CCl4-induced hepatotoxicity in vivo in rats and CCl4-induced cytotoxicity in isolated rat hepatocytes were investigated. Rats received different concentrations of the extract by i.p. injection for 3 consecutive days before the injection of (3ml/kg) CCl4 (i.p.). Twenty four ...
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